x

Adeola Folasade EHIGIE

Paste your official picture here (Not more than 200kb and 600px by 600px. Allowed types: png gif jpg jpeg. Your official picture
Contact Info
Verification Status
Admin:  
Pending
HR:  
Pending
Dr(Mrs) EHIGIE Adeola Folasade
Reader/Associate Professor
Female
Faculty: Faculty of Basic Medical Sciences
Department: Department of Biochemistry
Directorate/Unit:

Brief about me

I teach and research in the field of Biochemistry and specifically concentrating on Protein Science and Membrane Biochemistry. In our Labs, we do basic cell biochemistry studies looking closely at the intrinsic type of apoptosis which occurs via the modulation of the mitochondrial membrane permeability transition pore (MMPTP), a non-specific protein complex whose transition between the open and close state could mean live or death for a cell. Because of the ability of the pore to play this role of committing a cell to death or not, it has applications in the development of drugs that could treat diseases of cell proliferations such as various cancers and those useful in the treatment of diseases arising from cell depletion such as diabetes mellitus. In developing likely drugs we utilize known medicinal plants which active phytochemicals are then molecularly docked with known ligands (any of the protein components of the pore, especially cyclophylin D) to verify interactions. We also do apoptotic assays and cancer cell cytotoxic assays to confirm the mechanisms of action of discovered bioactive agents.

Academic Qualifications
1. General Certificate Education (GCE), 1994
2. Senior School Certificate of Education (SSCE), 1995
3. Bachelor of Science (B.Sc.) Biochemistry, 2001
4. Master of Science (M.Sc.) Biochemistry, 2004
5. Master of Philosophy (M. Phil.) Biochemistry, 2011
6. Doctor of Philosophy (PhD) Biochemistry, 2016

Professional Qualifications
1. International Computing Driving License (LCDL Africa) Short Course Training, Oct., 2020.
2. African Center of Excellence for Drug Research, Herbal Medicine Development and Regulatory Science (ACEDHARS) Short Course Training (Equivalent to 5.0. Continuing Education Units, Executive and Professional Education) on Toxicological Evaluation of Her

Academic Networking
Google Scholar URL
https://scholar.google.com/citations?user=69P8A5AAAAAJ&hl=en
LinkedIn URL
https://www.linkedin.com/in/adeola-folasade-ehigie-a435105b/
ResearchGate URL
https://www.researchgate.net/profile/Adeola_Odewusi-Ehigie
ORCID URL
https://orcid.org/0000-0001-9353-1123
Skills
1. Mitochondrial membrane permeability transition pore modulation assays
2. Cell culturing and sub-culturing assays
3. Cell viability assays
4. Cell membrane integrity assays
5. Western blotting
6. Mitochondrial membrane depolarization assays
7. Luciferin-luciferase assays
8. Lipid peroxidation assays
9. Flow cytometric assays
10. Microscopic assays (especially fluorescence)
11. Apoptosis assays
12. Protein determination assays
13. Reactive oxygen species generation assays
14. Molecular docking and simulations

Interests
• Membrane Biochemistry and Biophysics
• Medicinal plants
• Drug development
• Cancer research
• Computational Biochemistry; In silico studies and Molecular docking
• Protein Biochemistry

Languages
• Yoruba; English and Hausa

List of Publications
  1. Olorunnisola, O.S., Abbiyessiku, F.M., Kuti, B., Annetor, I., Odewusi, A.F., Olabinrin, B.M., Olaiya C.O. and Oladipo, I.C. (2007). Studies on Plasma Vitamins C, E and Glycated Plasma Haemoglobin Levels in Poorly and Well Controlled Type-2 Diabetic Patients in Western Nigeria. Science focus; 12:120-124.
  2. Afolabi, O.K, Adedosu, O.T, Adedeji, A.L, Odewusi A.F, Olorunsogo O.O (2007). Additive Effect of Lead on Calmodulin Stimulation of the Erythrocyte Ca2+ Mg2+ ATpase activities. Journal of Science and Technology Research 6:59-63. 
  3. Ehigie, L. O., Odewusi A.F, and Duszenko, M. (2007). The Usefulness of Proteomics in Protein Identification. Science focus; 12:27-36.
  4. Ehigie L. O., Odewusi A.F, Afolabi O.K, Olabinri B.M and Duszenko M. (2007). The Usefulness of Proteomics in the Identification of a Protein from the Causative Agent of Human African Trypanosomaisis: Trypanosoma brucei. International Journal of Molecular Medicine and Advance Sciences 3:155-159.
  5. Odewusi, A.F., Oyeyemi, M.O., Olayemi F.O., Emikpe, E., Ehigie, L.O., Adisa, R.A. and Olorunsogo O.O. (2010). Effects of the leaf decoction of Momordica charantia (bitter melon) on Mitochondrial Membrane Permeability Transition pore and fertility in normal male albino rats. African Journal of Medicine and Medical Sciences; 39(Suppl.):45-57.
  6. Ehigie, L. O., Ehigie, A.F, Olurunnisola, O.S. Olabinri, B.M, Adedeji, A.L. and Duszenko, M. (2010). Identification of Prostaglandin D in Trypanosoma brucei using co-immunoprecipitation methods. Science Focus; 15:371-378.
  7. Ehigie, L. O., Okonji, R. E, Ehigie A.F and Agboola F. K. (2010). Properties of Arginase from the Hepatopancreas of Giant Freshwater Prawn (Macrobrachium rosenbergii, de Man); Cameroon Journal of Experimental Biology; 6: 81-90.  
  8. A. F. Ehigie, L. O. Ehigie, S. A. Odediran, O. K. Afolabi, O. T. Adedosu, and O. O. Olorunsogo (2013). In vitro Induction of rat liver mitochondrial membrane permeability transition pore opening by solvent extracts of Momordica charantia leaves. Biokemistri; 25 (2): 52-60.
  9. L. O. Ehigie, R. E. Okonji and A. F. Ehigie (2013). Biochemical properties of thiaminase, a toxic enzyme in the gut of Grasshoppers (Zonocerus variegates Linn). Cameroon Journal of Experimental Biology. 09 (01): 9-16.
  10. O. T. Adedosu, A. T. Oyedeji, T. Iwakun, A. F. Ehigie and O. O. Olorunsogo (2014). Hepatoprotective Activity and Inhibitory Effect of Flavonoid-Rich Extract of Brysocarpus Coccineus Leaves on Mitochondrial Membrane Permeability Transition Pore. Asian Journal of Natural & Applied Sciences. 3(3):92-100.
  11. A. F. Ehigie, L. O. Ehigie, O. O. Alao, G. N. Anyasor, O. T. Oyebode and O. O. Olorunsogo (2014). Inhibitory Effect of The Methanol Leaf Extract of Momordica charantia on Ca2+-Induced Liver Mitochondrial Permeability Transition Pore Opening in Normal Rats. TWOWS (OWSDW) Africa International Journal of Science and Technology. 5 (1&2) 1– 10.
  12. Okonji, R. E., Ehigie, L. O., Popoola, M. O., Ehigie, A. F. and Komolafe, O. O. (2014). Physicochemical properties of Liver Arginase from Heterotis niloticus (Cuvier 1829). Journal of Science Research. 13; 45-56.
  13. Ehigie, L. O., Okonji, R. E., A. F. Ehigie, Adeoye, A. O. and Falode, J. A. (2015). Properties of Thiaminase in Fresh water Prawn Machrobrachium Rosenbergii. IMPACT: International Journal of Research in Applied, Natural and Social Sciences.3(4):37-48.
  14. L. O. Ehigie, R. E. Okonji, A. F. Ehigie, O. O. Ajayi and B. S. Fagbohunka (2015). Purification and characterization of Rhodanese from the Leaves of Bitter melon (Mormodica charantia). International Journal of Research in Applied, Natural and Social Sciences. 3(5):47-58.
  15. A. F. Ehigie, P. Wei, L. O. Ehigie, S. A. Odediran, T. Wei, X. Yan and O. O. Olorunsogo (2016). Cytotoxicity of fractions derived from Crude Water-Soluble Extract (CWSE) of Momordica charantia (Linn) Leaves on Lung, Cervical and Breast cancer cells. IRA-International Journal of Applied Sciences, 4(2), 296-304.
  16. Okonji, R. E., Ayanwusi O., Ehigie, A. F. and Ehigie, O. L. (2017). Biochemical Characterization of Alpha-amylase from the adult of Rhinoceros Beetle, (Scarabaeidae dynastinae) Acta Velit.  Vol 4 issue (2), 69 – 84.
  17. Ehigie, A. F., Okonji, R. E. and Ehigie, L. O. (2017). Biochemical Characterization of arginase from wood degrading termites. Acta Velit. Vol 4 (2), 58 – 68.
  18. A. F. Ehigie, M. A. Abdulrasak, L.O. Ehigie (2018) Biochemical Properties of Rhodanese from Almond (Prunus amygdalus) Nuts PAJOLS.
  19. A. F. Ehigie, O. O. Nwaechefu, G. N. Anyasor, F. S. Oyelere, T. S. Sangodipe and L. O. Ehigie (2018). Comparative Study of the Effects of Different Fractions of Ficus Exasperata Vahl on Rat Hepatic Mitochondrial Membrane Permeability Transition Pore. IRA-International Journal of Applied Sciences, 12(3), 15-29.
  20. A. F. Ehigie, R. E. Okonji, F. D. Ojeniyi and L. O. Ehigie (2018). Physiochemical properties of 3-mercaptopyruvatesulphurtransferase from Ginger (Zingiber Officinale). Asian Journal of Biochemical and Pharmaceutical Research.  Issue 1(Vol. 9) 119-130. India, Impact Factor; NIL.
  21. TA Oyedeji, AF Ehigie, OO Nwaechefu (2018). Antioxidant potential of methanol extract of Xylopia aethiopica leaves. Unilag J. Bas. Med. Sci. 6(11): 63-70.
  22. A. F. Ehigie, R. E. Okonji, W. A. Oladiran and L. O. Ehigie (2018). Isolation and Characterization of Rhodanese from Bacillus licheniformis Obtained from Industrial Effluents of Steel Rolling Industry in Ilorin, IRA-International Journal of Applied Sciences. Vol.13, (02), 14-34.
  23. A. F. Ehigie, M. A. Abdulrasak, F. D. Ojeniyi and L. O. Ehigie (2019).  Kinetic properties of Rhodanese from African locust bean seeds (Parkia biglobosa).  Asian Journal of Biomedical and Pharmaceutical Sciences Vol. 9 (67) 18-25.

  24.  Ehigie AF, Abdulrasak MA, Adeleke GE, Ehigie LO (2019). Comparison of Rhodanese Activity and Distribution in Tomato (Solanum lycopersicum Mill.) Plant Parts and its Physicochemical Characterization. Journal of Plant Biochemistry & Physiology Vol 7, (3) 1-8,

  25. A. F. Ehigie, G. E. Adeleke, W. A. Oladiran and L. O. Ehigie (2019). “​Screening for Rhodanese Producing Bacterium in Freshly Pressed Cassava Effluents of a Cassava Processing Industry Channeled to Odo-Oba Stream in Ogbomoso-Nigeria”, Journal of Applied & Natural Science 11(3): 650-656
  26. A. F. Ehigie, L. O. Ehigie, O. J. Olanlokun, F. S. Oyelere, O. T. Oyebode and O. O. Olorunsogo (2019). Inhibitory effect of the methanol leaf extract of Mormodica charantia on liver mitochondrial permeability transition pore opening in alloxan induced diabetic rats. European Journal of Biomedical and Pharmaceutical Sciences, Vol 6(8): 73-78.
  27. M. A. Abdulrasak, A. F. Ehigie and L. O. Ehigie (2019).  Comparative evaluation of poultry manure and NPK fertilizer on hydrogen cyanide concentration, total protein and Rhodanese activity in tomato (solanum lycopersicum l.) leaves. European Journal of Biomedical and Pharmaceutical sciences. Vol 6 (8), 437-442.

  28. *A. F. Ehigie, O. O. Alao, T. A. Oyedeji, F. S. Oyelere, L. O. Ehigie, and Olorunsogo O. O. (2019). Inductive Effects of Fractions Derived from the Crude Water-Soluble Extract of Momordica charantia on Rat Liver Mitochondrial Membrane Permeability Transition Pore. African Journal of Medicine and Medical Sciences; September issue, Vol 40 (3).

  29. Ojeniyi F.D., Ehigie A.F., Ehigie O.L., (2019). Evaluation of Enzymatic Changes in Sub lethal Thiosulphate Cyanide Poisoning Wistar Rats Treated with Chromolaena odorata (Linn.) and Sodium. Journal of Plant Biochemistry and Physiol. 7 (4) pp. 242.

  30. *Ehigie, A. F., Adeleke, G. E., Ojeniyi, F. D., and Ehigie, L. O. (2020). Bioefficiency of Chromolaena odorata    (Linn.) on hematological and lipid profiles in sublethal cyanide poisoning in male wistar rats. Journal of Applied and Natural Science, 12(1), 13-18.

  31. *A. F. Ehigie, R. E. Okonji, F. D. Ojeniyi, M. O. Jesulade, L. O. Ehigie. (2020). Physicochemical Properties of 3-Mercaptopyruvate Sulfurtransferase: A Cyanide Detoxifying Enzyme from the Hemolymph of Limicolaria flammae (Garden Snail). Pan African Journal of Life Sciences, 4(2): 68-76.

  32. G. E. Adeleke, O. T. Adedosu, L. Ehigie , P. I. Adegbola, A. A. Olaniyi, T. O. Agoola, A. F. Ehigie. (2020). Chemical constituents of water hyacinth (Eichhornia crassipes) methanol leaf extract and its effect on selected enzymes of Periplaneta Americana. Journal of Biotechnology, Computational Biology and Bionanotechnology BioTechnologia, Vol. 101 (4) 323–336.
  33. A. F. Ehigie, P. Wei, T. Wei, X. Yan, F. Ojeniyi, and L. Ehigie. (2021). Momordica charantia L. induces non-apoptotic cell death in human MDA-MB-436 breast and A549 lung cancer cells by disrupting energy metabolism and exacerbating reactive oxygen species’ generation. Journal of Ethnopharmacology. 277 (2021) 114036.   
  34. F. D. Ojeniyi, A. F. Ehigie, Aluko, O. L., Adeleke, G. E., and Ehigie, L. O. (2021). Effect of Hexane Extracts of Chromolaena odorata (Linn.) on Hematotoxicity Induced by Cyanide in Male Albino Wistar Rats. Asian Journal of Biochemistry, Genetics and Molecular Biology, 36–43.
  35. Ehigie, A. F., Akinyemi, O. A.,   Ayoade T. E., Ogundola A. F., Oyelere S. F.,  Olabinri P. F. (2021). Evaluation of the Antioxidant Potential of Aqueous Extracts of Moringa oleifera Leaf and Cocos nucifera Husk: A Comparative Analysis. Pharmaceutical and Biomedical Research. 7(4):295-302.
  36. Agboola, J. B., Ehigie, A. F., Ehigie, L. O., Ojeniyi, F. D., and Olayemi, A. A. (2022). Ameliorative role of Syzygium aromaticum aqueous extract on synaptosomal tyrosine hydroxylase activity, oxidative stress parameters, and behavioral changes in lead-induced neurotoxicity in mice. Journal of Food Biochemistry.
  37. S. A. Odediran, A. F. Ehigie, S. O. Akadiri, R. A. Bamikole, R. Adeniyi, R. Adeoti and J. A. Aladesanmi. (2022). In vivo Chemosuppressive effects of Combinations of Three Nigerian Ethnomedicinal Antimalarial Plants. European Journal of Medicinal Plants. 33 (2):14-24.
  38. I. D. Boyenle, A. K Oyedele, A. T. Ogunlana, A. F. Adeyemo, F. S. Oyelere, O. B. Akinola, T. I. Adelusi, L. O. Ehigie, A. F. Ehigie. (2022). Targeting the mitochondrial permeability transition pore for drug discovery: Challenges and opportunities. Mitochondrion 63 (2022) 57-71.
  39. A. F. Ogundola, C. Bvenura, A. F. Ehigie and A. J. Afolayan (2022). Effects of soil types on phytochemical constituents and antioxidant properties of Solanum nigrum. South African Journal of Botany. 151-325-333.
  40. O. K. Olarenwaju, L. O. Ehigie, S. F. Oyelere, T. A. Oyedeji, F. D. Ojeniyi, F. P. Olabinrin, I. F. Olaoye and A. F. Ehigie (2022). Fractions of Aqueous Extract of Plumbago zeylanica (L.) Root Induced Mitochondrial Membrane Permeability Transition Pore Opening in Rats. International Journal of Biochemistry Research & Review 31(7): 38-48.
  1. L. O. Ehigie, F. D. Ojeniyi, A. Aborisade, S. Nassar, C. Igbeneghu, J. Busayo Agboola and Adeola F. Ehigie (2022). Transcriptomic Analysis of Differential Gene Expression in Staphylococcus aureus-Induced Pneumonia in Pediatrics Based on Microarray Analysis. European Journal of Pharmaceutical and Medical Research. 9 (10):75-85.
  1. L. O. Ehigie, F. D. Ojeniyi, R. Adesina, C. Igbeneghu, S. Nassar and A. F. Ehigie (2022). Molecular Docking and Molecular Dynamics Simulation to Investigate Protein-ligand Binding of Potential Drug Candidates from Vernonia amygdalina against Wilms’ Tumor 1 (wt1) Protein. European Journal of Biomedical and Pharmaceutical sciences. 9 (8) 499-514.